A novel antibody to oxidized phosphatidylethanolamine that is specific for amino groups modified by lipid peroxides

ABSTRACT In this study, a novel antibody to oxidized phosphatidylethanolamine (PtdEtn) was generated and characterized. Oxidized PtdEtn antiserum recognized PtdEtn and not unoxidized. PtdEtn by ELISA. The antibody also immunstained cells previously incubated with lipid peroxides (LOOH). This antibody recognized only high-molecular-weight epitopes in plasma. Because this antibody recognizes a specific amino group modification without the accompanying alterations in amino acids that are usually encountered when proteins are incubated with LOOH, it might be useful for the selective detection of such epitopes in cells, tissues, and lipoproteins.