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Updated: Jun 24, 2026
Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
Published on: March 13, 2020
A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from
Hetty C van den Broeck1, Antoine H P America, Marinus J M Smulders
1Plant Research International, Wageningen UR, PO Box 16, 6700 AA Wageningen, The Netherlands. hetty.busink@wur.nl
Abstract:
The detection, analysis, and quantification of individual celiac disease (CD) immune responsive gluten proteins in wheat and related cereals (barley, rye) require an adequate and reliable extraction protocol. Because different types of gluten proteins behave differently in terms of solubility, currently different extraction protocols exist. The performance of various documented gluten extraction protocols is evaluated for specificity and completeness by gel electrophoresis (SDS-PAGE), immunoblotting and RIDASCREEN Gliadin competitive ELISA. Based on these results, an optimized, two-step extraction protocol has been developed.
