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Updated: Jun 24, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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Novel method for high-throughput colony PCR screening in nanoliter-reactors.

Marcel Walser1, Rene Pellaux, Andreas Meyer

  • 1ETH Zurich, Institute of Process Engineering, BioProcess Laboratory (BPL), Zurich, Switzerland.

Nucleic Acids Research
|March 14, 2009
PubMed
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We developed a novel nanoliter-reactor technology for rapid DNA identification and sequencing. This cost-effective method efficiently isolates genetic markers, like microsatellites, for applications in plant research.

Area of Science:

  • Biotechnology
  • Genomics
  • Molecular Biology

Background:

  • High-throughput screening of DNA elements is crucial for genetic research and diagnostics.
  • Existing methods can be costly and time-consuming, limiting scalability.
  • There is a need for efficient, cost-effective technologies for identifying conserved DNA elements.

Purpose of the Study:

  • To introduce a novel technology for rapid identification and sequencing of conserved DNA elements.
  • To demonstrate the application of this technology for isolating microsatellites in cassava.
  • To highlight the scalability and cost-effectiveness of the developed system.

Main Methods:

  • Utilizing novel alginate-based nanoliter (nl)-reactors as self-contained reaction compartments.

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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Last Updated: Jun 24, 2026

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High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

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  • Performing microbial growth, lysis, thermocycling, and fluorescence-based screening within suspended nl-reactors.
  • Employing a suspension array system for space-effective and low-cost high-throughput processing.

    • Main Results:

    • Successfully isolated and sequenced 11 high-quality microsatellites for cassava polymorphism studies.
    • Achieved high-throughput screening of 20,000 clones within 2 days.
    • Demonstrated the scalability of the nl-reactor technology for potential throughputs exceeding 100,000 samples per day.

    Conclusions:

    • The nl-reactor suspension array technology offers a rapid, scalable, and cost-effective solution for DNA element identification and sequencing.
    • This technology can significantly complement existing deep-sequencing methods.
    • The system shows great promise for accelerating genetic research, particularly in plant science and polymorphism studies.