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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Transgenic Organisms00:53

Transgenic Organisms

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Transgenic Organisms00:53

Transgenic Organisms

Overview

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Related Experiment Video

Updated: Jun 24, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes
10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Overview: engineering transgenic constructs and mice.

Naoto Haruyama1, Andrew Cho, Ashok B Kulkarni

  • 1National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA.

Current Protocols in Cell Biology
|March 14, 2009
PubMed
Summary
This summary is machine-generated.

Genetically modified mouse models are crucial for understanding gene function and disease mechanisms. This review covers essential technologies for creating transgenic mice, a key gain-of-function approach in research.

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Published on: March 29, 2019

Area of Science:

  • Cell biology
  • Genetics
  • Animal models

Background:

  • Understanding genotype-phenotype relationships is vital for disease research.
  • Genetically manipulated mouse models are essential for studying whole-animal disease processes.
  • Recent discoveries in gene function have implications for animal models.

Purpose of the Study:

  • To review basic technologies for generating conventional transgenic mice.
  • To provide an overview of transgenic construct design.
  • To explain strategies for establishing transgenic mouse lines.

Main Methods:

  • Review of conventional transgenic mouse generation techniques.
  • Overview of transgenic construct design.
  • Strategies for identification and establishment of transgenic mouse lines.

Main Results:

  • Detailed review of transgenic mouse generation technologies.
  • Explanation of construct design and line establishment.
  • Discussion of common challenges in generating transgenic mice.

Conclusions:

  • Conventional transgenic mice are a fundamental gain-of-function tool.
  • Understanding these technologies is crucial for researchers.
  • The review covers historical and emerging techniques in the field.