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Neutrophil passage through isolated perfused rabbit lungs
H Schütte1, S Rosseau, D Walmrath
1Department of Internal Medicine, Justus-Liebig-University Giessen, Federal Republic of Germany.
The American Journal of Physiology
|October 1, 1991
Summary
This study investigated polymorphonuclear leukocyte (PMN) lung passage kinetics using isolated rabbit lungs. Findings reveal PMN transit is significantly affected by flow rate and pressure, offering insights into PMN-endothelial interactions.
Area of Science:
- Pulmonary Physiology
- Immunology
- Cellular Kinetics
Background:
- Polymorphonuclear leukocytes (PMNs) play a crucial role in lung immune responses.
- Understanding PMN kinetics within the lung vasculature is essential for studying inflammatory processes.
Purpose of the Study:
- To investigate the kinetics of polymorphonuclear leukocyte (PMN) passage through ex vivo isolated rabbit lungs.
- To elucidate the influence of physiological parameters on PMN lung transit.
Main Methods:
- Utilized ex vivo isolated and ventilated rabbit lungs perfused with buffer.
- Injected 111In-labeled rabbit or human PMNs and monitored their passage and washout using radioactivity counting and a sodium-iodide detector.
- Controlled perfusion flow rate, pulmonary venous pressure, and pre-incubated PMNs with endotoxin.
Main Results:
- A fraction of 16.6% of rabbit PMNs rapidly passed the lung, with an exponential washout (t50 = 8.1 min).
- Human PMNs exhibited a slightly longer transit time (t50 = 12.2 min).
- Reduced flow, increased venous pressure, and endotoxin pre-incubation significantly prolonged PMN lung transit times.
Conclusions:
- Single-pass PMN kinetics in isolated lungs correlate with in vivo findings.
- This model facilitates the study of PMN-endothelial interactions under standardized conditions.
- Physiological factors like flow rate and pressure critically modulate PMN lung passage.