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Ptpcd-1 is a novel cell cycle related phosphatase that regulates centriole duplication and cytokinesis

Doaa H Zineldeen1, Midori Shimada, Hiroyuki Niida

  • 1Department of Cell Biology and Biochemistry, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.

Biochemical and Biophysical Research Communications
|March 17, 2009
PubMed

Insights

Researchers identified protein tyrosine phosphatase domain containing 1 (Ptpcd1) as a novel mitotic phosphatase. Ptpcd1 plays a role in centrosome duplication and cytokinesis, interacting with polo-like kinase 1.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Mitosis requires precise regulation of protein phosphorylation by kinases and phosphatases.
  • While many mitotic kinases are known, phosphatases involved remain largely uncharacterized.

Purpose of the Study:

  • To identify novel phosphatases regulating mitosis.
  • To investigate the function of a newly identified phosphatase, Ptpcd1, in mitotic processes.

Main Methods:

  • Sequence homology analysis to identify potential phosphatases.
  • Immunofluorescence microscopy to determine protein localization.
  • Cell-based assays to assess effects of Ptpcd1 overexpression or depletion on mitosis, centrosome duplication, and cytokinesis.

Main Results:

  • Protein tyrosine phosphatase domain containing 1 (Ptpcd1) was identified as a novel mitotic phosphatase with homology to Cdc14.
  • Ptpcd1 localizes with the centrosome marker gamma-tubulin and prevents centrosome amplification.
  • Ptpcd1 interacts with polo-like kinase 1 (Plk1), rescues Plk1 depletion-induced prometaphase arrest, and causes aberrant cytokinesis upon overexpression.

Conclusions:

  • Ptpcd1 is a novel mitotic phosphatase involved in regulating centrosome duplication.
  • Ptpcd1 plays a critical role in cytokinesis, similar to Plk1.
  • Ptpcd1 functions in coordination with Plk1 during mitotic progression.

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