Related Experiment Video
Updated: Jun 24, 2026

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
Published on: May 19, 2016
Phosphorylation of CREB, a cyclic AMP responsive element binding protein, contributes partially to lysophosphatidic
Yong-Jun Kwon1, Yuanjie Sun, Nam-Ho Kim
1Department of Pharmacology, College of Medicine, Institute of Natural Medicine, Hallym University, Chunchon, Gangwon-do 200-702, South Korea.
Abstract:
Lysophospholipids regulate a wide array of biological processes including cell survival and proliferation. In our previous studies, we found that in addition to SRE, CRE is required for maximal c-fos promoter activation triggered by lysophosphatidic acid (LPA). c-fos is an early indicator of various cells into the cell cycle after mitogenic stimulation. However, role of CREB activation in LPA-stimulated proliferation has not been elucidated yet. Here, we investigate how LPA induces proliferation in Rat-2 fibroblast cell via CREB activation. We found that total cell number and BrdU-positive cells were increased by LPA. Moreover, levels of c-fos mRNA and cyclin D1 protein were increased via LPA-induced CREB phosphorylation. Furthermore, LPA-induced Rat-2 cell proliferation was decreased markedly by ERK inhibitor (U0126) and partially by MSK inhibitor (H89). Taken together, these results suggest that CREB activation could partially up-regulate accumulation of cyclin D1 protein level and proliferation of LPA-stimulated Rat-2 fibroblast cells.
Related Concept Videos
cAMP-dependent Protein Kinase Pathways
GPCRs Regulate Adenylyl Cylase Activity
Two...
Amplifying Signals via Second Messengers
Intracellular Signaling Cascades
IP3/DAG Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway

