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Updated: Jun 24, 2026

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
Published on: December 7, 2015
Voltage-gated proton channel is expressed on phagosomes
Yoshifumi Okochi1, Mari Sasaki, Hirohide Iwasaki
1Department of Integrative Physiology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
Abstract:
Voltage-gated proton channel has been suggested to help NADPH oxidase activity during respiratory burst of phagocytes through its activities of compensating charge imbalance and regulation of pH. In phagocytes, robust production of reactive oxygen species occurs in closed membrane compartments, which are called phagosomes. However, direct evidence for the presence of voltage-gated proton channels in phagosome has been lacking. In this study, the expression of voltage-gated proton channels was studied by Western blot with the antibody specific to the voltage-sensor domain protein, VSOP/Hv1, that has recently been identified as the molecular correlate for the voltage-gated proton channel. Phagosomal membranes of neutrophils contain VSOP/Hv1 in accordance with subunits of NADPH oxidases, gp91, p22, p47 and p67. Superoxide anion production upon PMA activation was significantly reduced in neutrophils from VSOP/Hv1 knockout mice. These are consistent with the idea that voltage-gated proton channels help NADPH oxidase in phagocytes to produce reactive oxygen species.
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