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Updated: Jun 24, 2026

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure
Published on: April 28, 2023
Nanoindentation of gold nanoparticles functionalized with proteins
Heeyeon P Wampler1, Albena Ivanisevic
1Department of Chemistry, Purdue University, West Lafayette, IN 47907, United States.
Abstract:
The hardness and Young's modulus of 10 and 20 nm gold nanoparticles (Au NPs) modified with bovine serum albumin and streptavidin were measured using a nanoindenter. The Au NPs were immobilized on a semiconductor surface through organic self-assembled monolayers. Changes in mechanical properties occurred when the Au NPs were immobilized on the surface. The hardness and Young's modulus were dependent on the size of the NPs, and the proteins on the particles showed highly plastic and elastic behavior compared to flat surfaces modified with self-assembled monolayers.

