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Updated: Jun 24, 2026
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
Application of 'next-generation' sequencing technologies to microbial genetics
Daniel MacLean1, Jonathan D G Jones, David J Studholme
1The Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom.
Nature Reviews. Microbiology
|March 17, 2009
Summary
New sequencing technologies rapidly assemble microbial genomes. This review covers high-throughput sequencing capabilities and data analysis options for extracting valuable microbial genomic information.
Area of Science:
- Microbiology
- Genomics
- Bioinformatics
Background:
- Advancements in sequencing technologies have dramatically reduced the time required for microbial genome assembly.
- High-throughput sequencing generates vast amounts of genomic data, presenting new challenges.
Purpose of the Study:
- To review the capabilities of modern high-throughput sequencing technologies.
- To discuss methodologies and informatics options for analyzing microbial genomic data.
Main Methods:
- Review of current high-throughput sequencing platforms.
- Analysis of bioinformatics tools and pipelines for genome assembly and data interpretation.
Main Results:
- High-throughput sequencing enables rapid microbial genome assembly, often within days.
- A variety of computational tools and strategies exist for extracting meaningful insights from sequencing data.
Conclusions:
- The rapid pace of technological advancement in sequencing requires continuous adaptation of analytical methods.
- Effective utilization of high-throughput sequencing data is crucial for advancing microbial genomics research.
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