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Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: Jun 24, 2026

The Colon-26 Carcinoma Tumor-bearing Mouse as a Model for the Study of Cancer Cachexia
08:55

The Colon-26 Carcinoma Tumor-bearing Mouse as a Model for the Study of Cancer Cachexia

Published on: November 30, 2016

Decrease in malonyl-CoA and its background metabolic alterations in murine model of cancer cachexia.

Alper Celik1, Yoshihiko Kano, Shingo Tsujinaka

  • 1Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan.

Oncology Reports
|March 17, 2009
PubMed
Summary

Cancer cachexia involves altered lipid metabolism. This study found decreased malonyl-CoA and increased SSAT in cachectic mice, suggesting accelerated lipolysis contributes to weight loss.

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Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation
07:00

Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation

Published on: August 23, 2018

Related Experiment Videos

Last Updated: Jun 24, 2026

The Colon-26 Carcinoma Tumor-bearing Mouse as a Model for the Study of Cancer Cachexia
08:55

The Colon-26 Carcinoma Tumor-bearing Mouse as a Model for the Study of Cancer Cachexia

Published on: November 30, 2016

Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation
07:00

Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation

Published on: August 23, 2018

Area of Science:

  • Biochemistry
  • Oncology
  • Metabolism

Background:

  • Cancer cachexia is a complex metabolic syndrome characterized by involuntary weight loss.
  • The specific enzymatic alterations driving lipid degradation in cancer cachexia remain incompletely understood.

Purpose of the Study:

  • To investigate the changes in enzymatic activities related to lipid metabolism in a mouse model of cancer cachexia.
  • To identify key enzymes and metabolites involved in accelerated lipolysis during cachexia.

Main Methods:

  • Transplantation of two colon 26 adenocarcinoma subclones (clone 20 potent cachexia inducer, clone 5 non-inducer) into CDF-1 male mice.
  • Analysis of liver tissue on day 14 post-tumor inoculation, including real-time PCR for SSAT expression and Western blotting for ACC.
  • Measurement of malonyl-CoA levels and assessment of indomethacin's effects on body weight, fat weight, food intake, and key enzyme expressions.

Main Results:

  • Mice bearing clone 20 exhibited significantly lower body weight, food intake, and epididymal fat pad weight compared to control and clone 5 groups.
  • Cachectic mice showed significantly increased spermidine/spermine N-1 acetyl transferase (SSAT) expression and decreased acetyl-CoA carboxylase (ACC) and malonyl-CoA levels.
  • Indomethacin treatment reversed cachexia-associated weight loss, reduced food intake, and normalized SSAT, ACC, and malonyl-CoA levels.

Conclusions:

  • Decreased malonyl-CoA levels, which normally inhibit fatty acid beta-oxidation, likely contribute to accelerated lipolysis in cancer cachexia.
  • Increased SSAT expression and reduced ACC activity further support enhanced fatty acid mobilization in this condition.
  • Targeting these metabolic pathways may offer therapeutic strategies for managing cancer cachexia.