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Published on: July 1, 2022
Protein profile of Nomuraea rileyi spore isolated from infected silkworm
Lvgao Qin1, Xiaoyong Liu, Jun Li
1Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, Jiangsu Province, People's Republic of China. qinlvgao@163.com
Abstract:
Nomuraea rileyi (N. rileyi) is the causative agent of the silkworm, Bombyx mori, green muscardine which can cause severe worldwide economical loss in sericulture. Little is known about N. rileyi at the protein level for this entomopathogenic parasite which belongs to the Ascomycota. Here, we employed proteomic-based approach to identify proteins of N. rileyi spores collected from the dead silkworm. In all, 252 proteins were separated by two-dimensional gel electrophoresis (2-DE), and were subjected to mass spectrometry (MS) analysis, 121 proteins have good MS signal, and 24 of them were identified due to unavailability of genomic information from N. rileyi. This data will be helpful in understanding the biochemistry of N. rileyi.
Insights
This study identifies proteins in Nomuraea rileyi (N. rileyi), a silkworm pathogen causing economic losses. The proteomic analysis provides foundational data for understanding N. rileyi biochemistry and developing control strategies.
Area of Science:
- Entomology
- Mycology
- Proteomics
Background:
- Nomuraea rileyi (N. rileyi) causes green muscardine disease in silkworms (Bombyx mori), leading to significant economic losses in sericulture.
- Limited knowledge exists regarding the proteome of N. rileyi, an entomopathogenic fungus belonging to the Ascomycota.
Purpose of the Study:
- To identify proteins present in N. rileyi spores using a proteomic approach.
- To lay the groundwork for understanding the biochemical mechanisms of N. rileyi.
Main Methods:
- Proteomic analysis of N. rileyi spores.
- Two-dimensional gel electrophoresis (2-DE) for protein separation.
- Mass spectrometry (MS) for protein identification.
Main Results:
- Separated 252 proteins using 2-DE.
- Achieved good MS signals for 121 proteins.
- Identified 24 proteins, with genomic information for N. rileyi being unavailable.
Conclusions:
- The proteomic data provides novel insights into the N. rileyi proteome.
- This study contributes essential biochemical information for N. rileyi research.
- The findings can aid in developing strategies to manage N. rileyi in sericulture.
