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Updated: Jun 24, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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Microfluidic chips for protein differential expression profiling.

Jenny M Armenta1, Abdulilah A Dawoud, Iulia M Lazar

  • 1Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.

Electrophoresis
|March 17, 2009
PubMed
Summary

Microfluidic-mass spectrometry (MS) offers a sensitive and efficient method for biomarker discovery. This technology integrates sample preparation and analysis, enhancing throughput for applications like breast cancer research.

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Area of Science:

  • Biomedical research
  • Proteomics
  • Analytical chemistry

Background:

  • Biomarker discovery is crucial for early disease detection and improved patient survival.
  • Advancements in sensitive, specific, and rapid bioanalysis methods are essential for progress.
  • Microfluidics technology offers integrated sample preparation, separation, and detection, enhancing throughput in proteomics.

Purpose of the Study:

  • To report the integration of microfluidic liquid chromatography (LC) devices with mass spectrometry (MS) detection.
  • To evaluate the applicability of this integrated system for biomarker screening in breast cancer cellular extracts.
  • To explore the potential of LC-ESI-MS chips for comparative proteomic analysis of isotopically labeled cell extracts.

Main Methods:

  • Integration of microfluidic LC devices with MS detection.

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High-throughput Protein Expression Generator Using a Microfluidic Platform
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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Last Updated: Jun 24, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

  • Application to biomarker screening using MCF-7 breast cancer cellular extracts.
  • Proteomic analysis of approximately 0.1-1 microg of crude protein extract tryptic digest on-chip.
  • Exploration of LC-ESI-MS chips for comparative proteomic analysis of isotopically labeled MCF-7 breast cancer cell extracts.

    • Main Results:

    • Reliable identification of approximately 40-100 proteins from MCF-7 breast cancer cellular extracts.
    • Demonstration of microfluidics-MS as a competitive technology for biomarker discovery.
    • Successful application of LC-ESI-MS chips for comparative proteomic analysis.

    Conclusions:

    • Microfluidics-MS is a powerful and competitive technology for biomarker discovery and screening.
    • The integrated LC-microchip-MS system enables efficient proteomic analysis of complex biological samples.
    • This approach shows significant potential for advancing breast cancer research and diagnostics.