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Updated: Jun 24, 2026
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
Published on: January 21, 2020
[Mass chromatographic analysis on different protein expression of human periodontal ligament cell under static
Yuan-Yuan An1, Hong Zhou, Yu-Song Ruan
1Department of Orthodontics, Medical School of Xi'an Jiaotong University, Xi'an 710004, China. ann@stu.xjtu.edu.cn
Purpose:
To analyze the protein expression of human periodontal ligament cells (HPDLCs) under static pressure.
Methods:
The proteins of two groups of human periodontal ligament cell (one group was interfered by static pressure and one group was not) were analyzed by two-dimensional electrophoresis and mass chromatographic analysis. The silver stained proteins spots were analyzed by Image Master 2D Platinum Software 5.0. There were about 720 and 730 detectable spots on the two 2D-gels separately and nearly 30 spots which were differentially expressed.
Results:
With direct MALDI-TOF mass spectrometry and protein database searching, 2 protein spots out of 5 new appeared spots were identified. Presenilin 2 and catechol O-methyltransferase were expressed only in the group which was interfered by static pressure.
Conclusions:
The results of mass chromatographic analysis demonstrate that Notch pathway and calcitonin gene-related peptide(CGRP) may be the partners of the channel of biomechanical signal conduction of the periodontal ligament cell.