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Fluid dynamics in bioreactor design: considerations for the theoretical and practical approach
B Weyand1, M Israelowitz, H P von Schroeder
1Department of Plastic, Hand and Reconstructive Surgery OE 6260, Hannover Medical School, Carl-Neubergstr. 1, Hannover, 30625, Germany.
Advances in Biochemical Engineering/Biotechnology
|March 18, 2009
Summary
This chapter covers fluid dynamics principles crucial for designing bioreactors, especially for mammalian cell cultures. Understanding these concepts optimizes cell culture system performance and efficiency.
Area of Science:
- Biotechnology
- Chemical Engineering
- Cell Biology
Background:
- Bioreactor design is critical for successful mammalian cell culture.
- Fluid dynamics significantly impacts cellular environment and process outcomes.
- Optimizing bioreactors requires a deep understanding of fluid behavior.
Purpose of the Study:
- To summarize key fluid dynamics principles relevant to bioreactor design.
- To highlight the application of these principles in mammalian cell-culture systems.
- To provide a foundation for engineers and scientists involved in bioreactor development.
Main Methods:
- Review of fundamental fluid dynamics concepts.
- Application of principles to bioreactor geometries and operations.
- Case examples focusing on mammalian cell culture.
Main Results:
- Identification of critical fluid dynamic parameters (e.g., mixing, shear stress, mass transfer).
- Correlation between fluid dynamics and cell viability, growth, and productivity.
- Guidelines for designing bioreactors to achieve desired hydrodynamic conditions.
Conclusions:
- Effective bioreactor design for mammalian cell culture necessitates careful consideration of fluid dynamics.
- Tailoring hydrodynamic conditions can enhance cell performance and product yield.
- Further research can refine design strategies based on fluid dynamic modeling.
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