Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 24, 2026

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts
10:32

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts

Published on: March 26, 2015

Anatomic double-bundle versus single-bundle ACL reconstruction: a comparative biomechanical study in rabbits.

Vassilios S Nikolaou1, Nicolas Efstathopoulos, Ioannis Sourlas

  • 12nd Academic Department of Trauma and Orthopaedics, School of Medicine, Athens University, Megalou Alexandrou 54, 15124 Maroussi, Athens, Greece. vassilios.nikolaou@gmail.com

Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
|March 18, 2009
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adenoma detection rates during screening colonoscopy performed by interventional gastroenterologists and general gastroenterologists.

iGIE : innovation, investigation and insights·2026
Same author

Response.

Gastrointestinal endoscopy·2026
Same author

International Multidisciplinary Consensus Report on Definitions, Diagnostic Criteria, and Management of Fatty Pancreas: A Joint Statement Endorsed by EPC, APA, EASD, EASL, ESGAR, ESGE, ESP, ESPCG, ESPEN, ESPGHAN, IAP, JPS, KPBA, LAPSG, and UEG.

United European gastroenterology journal·2026
Same author

Neuromuscular Scoliosis as a Rare Manifestation of Guillain-Barré Syndrome in a Child.

Cureus·2026
Same author

Alumina Production from Calcium Aluminate Slags with a Novel HCl-Based Metallurgical Process.

Journal of sustainable metallurgy·2025
Same author

Surgical Management of Pediatric Capitellar Fractures: A Case Series of Five Patients.

Cureus·2025

This study compared two surgical methods for repairing torn knee ligaments in rabbits. Researchers found that a double-bundle technique provided better knee stability than a single-bundle approach when measured at specific angles. These findings help clarify how different surgical designs affect joint movement and recovery.

Area of Science:

  • Orthopedic surgery and biomechanical research within musculoskeletal medicine
  • Anatomic double-bundle ACL reconstruction techniques for joint stability

Background:

No prior work had resolved whether dual-strand ligament repairs offer superior mechanical advantages over traditional single-strand methods in animal models. Surgeons frequently debate which graft configuration best restores natural knee kinematics after injury. That uncertainty drove the need for controlled experiments comparing these specific surgical approaches. Prior research has shown that ligamentous integrity remains a primary concern for long-term joint health. This gap motivated an investigation into how graft placement influences tibial translation during flexion. Investigators often utilize rabbit models to simulate human ligamentous conditions due to anatomical similarities. Understanding these biomechanical differences remains a priority for improving surgical outcomes in clinical practice. This study addresses the mechanical performance of two distinct reconstruction strategies using standardized testing protocols.

Purpose Of The Study:

The aim of this study was to compare the biomechanical performance of two different ligament reconstruction techniques in a rabbit model. Researchers sought to determine if a dual-strand approach provides better joint stability than a traditional single-strand method. The study addressed the need for objective data regarding how graft placement influences postoperative knee kinematics. No prior work had fully characterized the mechanical advantages of these specific configurations in this animal model. That uncertainty drove the team to quantify anterior tibial shift at multiple flexion angles. Investigators hypothesized that the dual-strand design would more effectively mimic natural ligamentous function. This research provides a controlled environment to evaluate the efficacy of complex surgical graft placements. The findings contribute to the ongoing discussion regarding optimal reconstruction strategies for restoring joint integrity after injury.

Keywords:
orthopedic biomechanicstibial translationjoint stabilityligament repair

Frequently Asked Questions

The researchers propose that the double-bundle technique provides superior stability by reducing anterior tibial translation. Specifically, at ninety degrees of flexion, the dual-strand method resulted in a 1.92 mm smaller anterior shift compared to the single-strand procedure.

The investigators utilized a custom-modified device modeled after a KT1000 arthrometer. This apparatus incorporated a Mitutoyo dial indicator 2050 to quantify precise displacement measurements during the biomechanical assessments.

Testing at both thirty and ninety degrees of flexion was necessary to evaluate joint stability across different ranges of motion. These specific angles allowed the team to assess how the graft configuration performs under varying degrees of mechanical stress.

The study utilized New Zealand white rabbits as the experimental model. Thirty animals were divided into two groups, with fifteen receiving the dual-strand repair and fifteen receiving the single-strand autograft.

More Related Videos

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit
06:11

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit

Published on: May 14, 2020

Related Experiment Videos

Last Updated: Jun 24, 2026

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts
10:32

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts

Published on: March 26, 2015

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit
06:11

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit

Published on: May 14, 2020

Main Methods:

The review approach involved a comparative analysis of two distinct surgical reconstruction protocols in thirty New Zealand white rabbits. Researchers performed ligament repairs using either a dual-strand or single-strand graft configuration. The team utilized the medial third of the patellar tendon and semitendinosus tendon for the dual-strand group. Conversely, the single-strand group received only a semitendinosus tendon autograft. Investigators assessed joint stability using a modified arthrometer equipped with a high-precision dial indicator. Measurements occurred at thirty and ninety degrees of flexion during three distinct study phases. These phases included preoperative assessment, post-resection evaluation, and a final check three months after the operation. Statistical comparisons determined the significance of differences in anterior tibial translation between the two cohorts.

Main Results:

Key findings from the literature demonstrate that the dual-strand technique significantly improves knee stability at ninety degrees of flexion. The mean anterior shift for this group was 1.92 mm lower than the single-strand group. This specific difference reached statistical significance with a P-value of 0.006. At thirty degrees of flexion, the dual-strand method also showed a smaller mean anterior shift. This reduction measured 0.66 mm compared to the single-strand procedure. However, this specific difference did not reach the threshold for statistical significance. The data indicate that the dual-strand approach provides a more stable joint regarding anterior tibial movement. These results highlight the mechanical performance differences between the two surgical strategies tested in this model.

Conclusions:

The authors report that the dual-strand surgical approach provides enhanced stability compared to the single-strand method. Their data indicate a significant reduction in anterior tibial translation at ninety degrees of flexion. No statistical significance was observed regarding stability improvements at thirty degrees of flexion. These results suggest that graft configuration influences mechanical outcomes differently depending on the joint angle. The researchers propose that their animal model serves as a viable platform for future investigations. Future work might explore additional variables that impact the success of these complex ligament repairs. This synthesis implies that anatomical reconstruction designs may offer functional benefits for joint stabilization. The study provides evidence supporting the potential utility of dual-strand techniques in orthopedic applications.

The team measured the mean anterior shift of the tibia relative to the femur. They compared the displacement values between the two surgical groups at three months postoperatively to determine the efficacy of each reconstruction method.

The authors suggest that this animal model could be useful for examining other factors affecting surgical success. They propose that future studies might investigate additional parameters that influence the long-term outcomes of these complex ligament repairs.