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Updated: Jun 24, 2026

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Functionalized nanoparticles for nucleic acid sequence analysis using optical spectroscopies.

Duncan Graham1, Karen Faulds, David Thompson

  • 1Department of Pure and Applied Chemistry, Centre for Molecular Nanometrology, WestCHEM, University of Strathclyde, Glasgow, UK. duncan.graham@strath.ac.uk

Biochemical Society Transactions
|March 18, 2009
PubMed
Summary
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Surface-enhanced resonance Raman scattering (SERRS) offers highly sensitive DNA detection, surpassing fluorescence. This study demonstrates quantitative SERRS for labeled DNA probes and sequence identification via hybridization-induced nanoparticle aggregation.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Molecular Diagnostics

Background:

  • Surface-enhanced resonance Raman scattering (SERRS) is a highly sensitive vibrational spectroscopy technique.
  • SERRS requires a visible chromophore and an enhancing surface, typically metallic nanoparticles, for DNA detection.
  • DNA lacks inherent SERRS activity due to the absence of a suitable chromophore and poor surface adsorption.

Purpose of the Study:

  • To demonstrate quantitative detection of labeled DNA probes using SERRS with commercially available labels.
  • To develop a SERRS-based method for DNA sequence identification by exploiting nanoparticle aggregation.
  • To explore the potential of SERRS for closed-tube molecular diagnostics.

Main Methods:

  • Modification of DNA probes with SERRS labels.

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  • Utilizing metallic nanoparticles as the enhancing surface.
  • Exploiting DNA hybridization to induce nanoparticle aggregation and activate the SERRS signal.

    • Main Results:

    • Achieved straightforward and quantitative detection of labeled DNA probes using SERRS.
    • Demonstrated DNA sequence identification through SERRS signal generation triggered by hybridization-induced aggregation.
    • Showcased the critical role of nanoparticle aggregation state in SERRS sensitivity.

    Conclusions:

    • SERRS provides highly sensitive and selective DNA detection, rivaling and often surpassing fluorescence.
    • The developed SERRS approach enables quantitative analysis and sequence-specific identification of DNA.
    • SERRS, particularly with aggregation-based detection, shows significant promise for molecular diagnostics.