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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
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Genomic DNA amplification by the multiple displacement amplification (MDA) method.

Roger S Lasken1

  • 1J Craig Venter Institute, La Jolla, CA 92121, USA. rlasken@jcvi.org

Biochemical Society Transactions
|March 18, 2009
PubMed
Summary

Multiple displacement amplification (MDA) generates large DNA amounts from minimal samples, overcoming limitations in genomic analysis and detection assays. This method offers extensive genome coverage and high yields, even from single cells.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Limited DNA availability hinders detection assays and genomic analysis.
  • Small specimens or environmental samples often contain low DNA content.
  • Whole genome amplification is crucial for generating sufficient DNA material.

Purpose of the Study:

  • To discuss the utility of Multiple Displacement Amplification (MDA) for DNA amplification.
  • To highlight MDA's advantages over older whole genome amplification methods.
  • To explore the applications, strengths, and limitations of MDA.

Main Methods:

  • Utilized Multiple Displacement Amplification (MDA) for whole genome amplification.
  • Compared MDA performance with older whole genome amplification techniques.
  • Evaluated DNA yields and product lengths from low-input DNA samples.

Main Results:

  • MDA provides extensive genome coverage.
  • MDA generates extremely long DNA products.
  • MDA achieves high DNA yields, even from minute starting material quantities.

Conclusions:

  • Multiple Displacement Amplification is an effective method for generating large DNA quantities.
  • MDA is suitable for applications requiring extensive genomic coverage and high yields.
  • MDA technology enables genomic analysis from single cells and low-biomass samples.