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Updated: Jun 24, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

[Not Available].

C Lafforgue¹, F Croute, J F Nicolas

¹Laboratoire de Dermopharmacie et Cosmétologie, Faculté de Pharmacie - 8 avenue Rockefeller - 69008 Lyon.

International Journal of Cosmetic Science

|March 18, 2009

Summary

This summary is machine-generated.

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Propionylaminoacid derivatives demonstrate significant antielastase activity, inhibiting both pancreatic and fibroblastic elastase. These compounds also enhance cellular respiration and show good in vitro tolerance for fibroblasts.

Area of Science:

Biochemistry
Dermatology
Cell Biology

Background:

Elastase enzymes play crucial roles in physiological processes, but their dysregulation is implicated in various pathological conditions.
Fibroblast function, including collagen production and cellular respiration, is vital for skin health and repair.
Understanding modulators of elastase activity and fibroblast metabolism is key to developing therapeutic interventions.

Purpose of the Study:

To investigate the antielastase activity of propionylaminoacid derivatives against pancreatic and fibroblastic elastase.
To evaluate the effects of these derivatives on essential metabolic functions of normal dermal fibroblasts.
To assess the in vitro safety and efficacy of these compounds at varying concentrations.

Main Methods:

Related Experiment Videos

Last Updated: Jun 24, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

Enzyme inhibition assays were performed to determine the antielastase activity of propionylaminoacid derivatives on pancreatic elastase.

Fibroblastic elastase production was measured.

In vitro studies on normal dermal fibroblasts assessed adhesion, proliferation, total protein and collagen (Type I and III) biosynthesis, and oxygen consumption.

Main Results:

Propionylaminoacid derivatives exhibited dose-dependent inhibition of pancreatic elastase (20-50% inhibition at 5-80 mg/ml).
Fibroblastic elastase production was inhibited, and cellular respiration was enhanced by the derivatives.
At concentrations up to 1 mg/ml, the derivatives showed excellent in vitro tolerance, with no adverse effects on fibroblast adhesion, proliferation, or collagen production, while further enhancing oxygen consumption.

Conclusions:

Propionylaminoacid derivatives possess significant antielastase properties and positively modulate fibroblast metabolic activity.
These findings suggest potential therapeutic applications for propionylaminoacid derivatives in conditions involving elastase activity and compromised skin health.
The compounds demonstrate a favorable safety profile in vitro, supporting further investigation for dermatological applications.