Determination of alpha-bisabolol and d-panthenol in cosmetic products by gas chromatography

Synopsis alpha-bisaboloi and d-panthenol are used in many cosmetic preparations, respectively, for their anti-inflammatory and regenerating properties. Their quantitative determination was an important element of their evaluation in these emulsions. Their concentration has been determined by gas chromatographic techniques, using a flame ionization detector. In both cases, the internal standards have been chosen for their compatibility with the analysis of extracted substances. Owing to the complexity of the cosmetic formulations, a preliminary extraction of alpha-bisabolol and d-panthenol was necessary. For the two substances the preparative separation was based on a liquid-liquid extraction. After dissolving the emulsion in methanol and diluting it with an aqueous buffer solution alpha-bisabolol was then extracted by ethyl acetate and d-panthenol by ethyl formate.