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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: Jun 24, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
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Published on: April 3, 2014

Dye labelling of nucleosides.

Alan R Katritzky1, Levan Khelashvili, Judit Kovacs

  • 1Center for Heterocyclic Compounds, Department of Chemistry, University of Florida, Gainesville, FL 32611-7200, USA. katritzky@chem.ufl.edu

Chemical Biology & Drug Design
|March 18, 2009
PubMed
Summary

Researchers synthesized novel dye-labeled nucleosides and threoninol conjugates using N-(4-arylazobenzoyl)-1H-benzotriazoles. These compounds were successfully created and characterized, opening avenues for new biochemical tools.

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Published on: July 6, 2016

Area of Science:

  • Organic Chemistry
  • Biochemistry
  • Synthetic Chemistry

Background:

  • Nucleoside analogs are crucial in medicinal chemistry and molecular biology.
  • Developing efficient methods for labeling biomolecules is essential for various applications.
  • Benzotriazole derivatives offer versatile reactivity for synthetic transformations.

Purpose of the Study:

  • To synthesize novel dye-labeled nucleosides and threoninol conjugates.
  • To explore the utility of N-(4-arylazobenzoyl)-1H-benzotriazoles as labeling agents.
  • To characterize the newly synthesized compounds.

Main Methods:

  • Treatment of N-(4-arylazobenzoyl)-1H-benzotriazoles (3a-b) with nucleosides.
  • Treatment of N-(4-arylazobenzoyl)-1H-benzotriazoles (3a-b) with threoninol.
  • Characterization of products using Nuclear Magnetic Resonance (NMR) spectroscopy and elemental analysis.

Main Results:

  • Dye-labeled nucleosides were obtained in 30-79% yields (average 45%).
  • Dye-labeled threoninol conjugates were synthesized in 55-89% yields (average 67%).
  • All synthesized compounds were confirmed through NMR and elemental analysis.

Conclusions:

  • N-(4-arylazobenzoyl)-1H-benzotriazoles are effective reagents for synthesizing dye-labeled nucleosides and threoninol conjugates.
  • The developed method provides a reliable route to novel labeled biomolecule derivatives.
  • These findings contribute to the toolkit for creating specialized biochemical probes.