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Updated: Jun 24, 2026
Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
Published on: January 25, 2017
[Study on the detection method for determining the bioactivity of recombinant human interleukin-22]
Jun-Yao Zhang1, Zhi-Hua Huang, Yu-Liang Huang
1Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To develop a method for determining the bioactivity of recombinant human IL-22.
Methods:
pSTAT3-TA-Luc and pcDNA3. 1 plasmids were co-transfect to HepG2 cells to generate stable HepG2/ STAT3 cell line with G418 screen. After treating cell with IL-22, the luciferase activity was assayed. Orthogonal test was used to optimize the assay condition, and then the reproducibility and specificity of the assay was checked.
Results:
The best condition for this assay are: cell density as 4 x 10(5)/mL, stimulating time as 4 hours, luciferase substrate as 50 microL. 50% effective dose (ED50) of IL-22 assayed by this method is 16.89 ng/mL, relative standard deviation (RSD) is 7.09%. Neutralizing antibody test shows the high specificity. Comparing with ELISA, the method described here has more advantages, including higher stability, easier performance and less cost.
Conclusion:
Luciferase reporter gene assay method is a fast, sensitive, reproducible method for IL-22 bioactivity determination.
