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11beta-hydroxysteroid dehydrogenase type 1 expression in periprosthetic osteolysis
Robert Z Tashjian1, Chuzhao Lin, Bassam Aswad
1Department of Orthopedics, University of Utah Orthopaedic Center, 590 Wakara Way, Salt Lake City, UT 84108, USA.
Orthopedics
|March 19, 2009
Summary
Periprosthetic membranes show increased 11Beta-hydroxysteroid dehydrogenase type 1 (11Beta-HSD1) expression. This may enhance local cortisol, potentially worsening osteolysis by inhibiting bone-forming cells.
Area of Science:
- Biochemistry
- Immunology
- Orthopedics
Background:
- Periprosthetic membranes contain fibroblasts, macrophages, and cytokines like IL-1Beta and TNF-alpha.
- Glucocorticoids may inhibit osteolysis by upregulating 11Beta-HSD1 in membrane fibroblasts.
Purpose of the Study:
- To evaluate the presence and expression of 11Beta-HSD1 in periprosthetic membranes.
- To investigate the role of IL-1Beta and TNF-alpha in regulating 11Beta-HSD1 in fibroblasts.
Main Methods:
- Immunohistochemistry was used to detect 11Beta-HSD1 in 15 periprosthetic membranes.
- Fibroblast cell cultures were treated with IL-1Beta and TNF-alpha to assess 11Beta-HSD1 gene expression.
Main Results:
- 11Beta-HSD1 was detected in all evaluated periprosthetic membranes.
- Fibroblast 11Beta-HSD1 gene expression was significantly upregulated following exposure to IL-1Beta and TNF-alpha.
Conclusions:
- Increased 11Beta-HSD1 expression in fibroblasts may elevate local cortisol levels within periprosthetic membranes.
- This elevation could exacerbate osteolysis by inhibiting osteoblastic function, contributing to implant loosening.
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