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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

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ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
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Published on: June 9, 2011

Microchip-based homogeneous immunoassay using fluorescence polarization spectroscopy.

Tomoya Tachi1, Noritada Kaji, Manabu Tokeshi

  • 1Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, 464-8603, Japan.

Lab on a Chip
|March 19, 2009
PubMed
Summary
This summary is machine-generated.

We developed a rapid microchip fluorescence polarization immunoassay (FPIA) for quick drug analysis. This homogeneous assay achieves quantitative results in about a minute, enabling faster diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Fluorescence polarization immunoassay (FPIA) is a homogeneous competitive immunoassay.
  • Conventional FPIA is used for laboratory tests but can be time-consuming.
  • Microchip-based assays offer potential for miniaturization and speed.

Purpose of the Study:

  • To realize a rapid fluorescence polarization immunoassay (FPIA) on a microchip.
  • To develop a microfluidic system for quick, quantitative analysis of small drug molecules.
  • To demonstrate the feasibility of microchip FPIA for point-of-care testing applications.

Main Methods:

  • Constructed a microfluidic FPIA system using a novel microchip, laser, CCD camera, and optical microscope with polarizers.
  • Utilized theophylline as a model analyte, introducing it and a fluorescently labeled tracer into a microchannel with antibody.
  • Investigated diffusion and mixing times within the microchannel to optimize assay performance.

Main Results:

  • Achieved quantitative analysis of theophylline in serum near therapeutic range in 65 seconds.
  • Demonstrated that tracer-antibody complex fluorescence polarization is inversely related to analyte concentration.
  • The microchip FPIA requires no separation processes, unlike heterogeneous immunoassays.

Conclusions:

  • Microchip-based FPIA is a simple and rapid immunoassay method.
  • This technology eliminates the need for washing, reflowing, or immobilizing steps.
  • Microchip FPIA holds significant promise for future point-of-care testing in clinical settings.