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[Determination of zizybeoside II of Ziziphus jujuba by HPLC]
Ji-Wei Niu1, Qi-Wei Zhang, Mu-Xin Gong
1College of Veterinary Medicine, Northwest Agricultural and Forest University, Yangling 712100, China.
Objective:
To develop a quantitative method for determination of zizybeoside II in Ziziphus jujuba.
Method:
The samples were separated at 30 degrees C on a Zorbax SB-C18 column eluted with methanol-water (20 : 80) as the mobile phase. Flow rate was set at 1.0 mL x min(-1) and the detection wavelength was set at 210 nm.
Result:
The calibration curve was linear within the range from 0.046 to 0.582 microg (r = 0.999 9) and the average recovery was 97.2%. 12 batches of the crude drugs purchased from different areas were determined and the contents of zizybeoside II in Fructus Jujubae were fluctuated from 0.013% to 0.041%.
Conclusion:
The method is simple, repeatable and could be used for the quality control of Z. jujuba.
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