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[Regulation function of Qingnao drop pilula to MARCKS mRNA express changes in acute cerebral ischemia hippocampus]
Yun-Ling Zhang1, Qi-Hui Zhang, Wen Bai
1Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China. yunlingzhang2004@yahoo.com.cn
Objective:
To observe the contribution of Qingnao drop pilula to the alteration of myristoylated alanine-rich C kinase substrate (MARCKS) mRNA expression in acute multi-infarction hippocampus.
Method:
Rat models of acute multi-infarction were established by injecting the embolus of blood powder through the right external carotid arteryinto the internal carotid artery, rats were randomly divided into five groups (n = 12 in each): normal, sham operation, model, Chinese medicine treatment, and Western medicine treatment. Qingnao drop pilula (133.28 mg x kg(-1)), nimodipine (7.25 mg x kg(-1)) were administered respectively to Chinese medicine treatment group and Western medicine treatment group by gavage, equal volume of normal saline were given to three groups. Rats were treated with drugs starting at 3rd day before the operation, one time per day. Observing morphologic changes in hippocampus by optical microscope and electron microscope. Detecting expression level of MARCKS mRNA in hippocampus by semi-quantification PCR method.
Result:
Hippocampus cells arrange tidy, administrative levels were compactness in normal group, which cells differentially impaired in model group, Chinese medicine treatment group and Western medicine treatment group. Hippocampus cells damage of Chinese medicine treatment group have more reckless than the model group in histopathology. The MARCKS mRNA were expressioned in model group vs medication treatment groups, in Chinese medicine treatment group vs the model group.
Conclusion:
Qingnao drop pilula can alleciate histomorphology lesion of hippocampus when occurring acute multi-infarction, to turn slower MARCKS mRNA expression, may play a neuroprotective effect role through accommodating PKC-MARCKS signal transduction system.
Insights
Qingnao drop pilula alleviates hippocampus damage in acute multi-infarction models. It slows myristoylated alanine-rich C kinase substrate (MARCKS) mRNA expression, suggesting a neuroprotective role.
Area of Science:
- Neuroscience
- Pharmacology
- Traditional Chinese Medicine
Background:
- Acute multi-infarction can lead to hippocampus damage.
- Myristoylated alanine-rich C kinase substrate (MARCKS) mRNA expression is altered in neurological conditions.
Purpose of the Study:
- To investigate the effect of Qingnao drop pilula on MARCKS mRNA expression in the hippocampus following acute multi-infarction in a rat model.
- To evaluate the neuroprotective potential of Qingnao drop pilula.
Main Methods:
- Rat models of acute multi-infarction were established.
- Rats were treated with Qingnao drop pilula, nimodipine, or saline.
- Hippocampus morphology was examined using light and electron microscopy.
- MARCKS mRNA expression was quantified using semi-quantitative PCR.
Main Results:
- Significant hippocampus cell damage was observed in the model group compared to the normal group.
- Qingnao drop pilula treatment showed some improvement in hippocampus histopathology compared to the model group.
- MARCKS mRNA expression patterns differed between treatment groups and the model group.
Conclusions:
- Qingnao drop pilula can alleviate hippocampus histomorphological lesions in acute multi-infarction.
- The medication appears to slow down MARCKS mRNA expression.
- Qingnao drop pilula may exert neuroprotective effects via the PKC-MARCKS signal transduction pathway.
