Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An uncommon life-threatening pyomyoma after repeated hysteroscopic procedures for abnormal uterine bleeding.

Taiwanese journal of obstetrics & gynecology·2026
Same author

An efficient and compact 1 × 8 beam splitter based on a tapered multimode Si waveguide at<i>λ</i><sub>0</sub>= 1330 nm.

Nanotechnology·2026
Same author

Dual PI3K/AKT/mTOR and CDK4/6 inhibition suppresses survivin to overcome uterine dedifferentiated endometrial carcinoma.

Cancer gene therapy·2026
Same author

Association of GJB2 P.V37I With Sudden Sensorineural Hearing Loss and Endoplasmic Reticulum Stress.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery·2026
Same author

Reply to: Comment on "Promising response to neoadjuvant pembrolizumab and low-dose chemotherapy in advanced endometrial cancer: A preliminary report".

Journal of the Formosan Medical Association = Taiwan yi zhi·2026
Same author

Hypomethylation of VTRNA2-1 promoter predicts adverse outcomes in peripheral artery disease.

Clinical epigenetics·2026

Related Experiment Video

Updated: Jun 24, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

Microarray labeling extension values: laboratory signatures for Affymetrix GeneChips.

Yun-Shien Lee1, Chun-Houh Chen, Chi-Neu Tsai

  • 1Department of Biotechnology, Ming Chuan University, Tao-Yuan, Taiwan.

Nucleic Acids Research
|March 20, 2009
PubMed
Summary

Labeling extension values (LEVs) correlate with gene expression levels (GEVs) in eukaryotic microarray data. LEVs can identify laboratory-specific variations, improving Affymetrix GeneChip data comparability.

More Related Videos

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Related Experiment Videos

Last Updated: Jun 24, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Interlaboratory comparisons of microarray data face challenges from RNA quality, labeling, hybridization, and data analysis.
  • Affymetrix GeneChips use multiple oligonucleotides to measure transcript levels, introducing potential variability.

Purpose of the Study:

  • To investigate the relationship between labeling extension values (LEVs) and gene expression levels (GEVs) in Affymetrix microarray data.
  • To determine if LEVs can identify laboratory-specific signatures and improve data comparability.

Main Methods:

  • Analysis of 2414 publicly available Affymetrix cel files across 20 microarray types and 13 species.
  • Calculation and correlation of LEVs (probe intensity vs. position) with GEVs.
  • Examination of LEVs for differentiating results from different laboratories and assessing their impact on biological process discovery.

Main Results:

  • LEVs are highly correlated with GEVs in eukaryotic Affymetrix microarray data, but not in prokaryotic data.
  • LEVs successfully differentiated results from the same specimens analyzed in different laboratories, revealing 'laboratory signatures'.
  • Filtering high-LEV genes did not hinder the identification of biological processes from differentially expressed genes.

Conclusions:

  • LEVs offer a novel filtering parameter for Affymetrix GeneChip microarray analysis.
  • LEVs have the potential to enhance both inter- and intralaboratory comparability of gene expression data.