Related Experiment Video
Updated: Jun 24, 2026
06:47
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
Published on: May 4, 2018
Bovine dentine organic matrix down-regulates osteoclast activity
Wantida Sriarj1, Kazuhiro Aoki, Keiichi Ohya
1Section of Developmental Oral Health Science, Department of Orofacial Development and Function, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Journal of Bone and Mineral Metabolism
|March 20, 2009
Summary
Dentine extracts from both baby and adult teeth can suppress osteoclast activity, which is crucial for root resorption. This suggests factors inhibiting bone remodeling are present in all tooth types.
Area of Science:
- Oral biology
- Cell biology
- Biochemistry
Background:
- Physiological root resorption occurs in deciduous (baby) teeth, while permanent teeth only resorb under pathological conditions.
- The molecular mechanisms driving root resorption in different tooth types remain largely unknown.
- Previous research indicated higher osteoclast resorption on deciduous dentine compared to permanent dentine.
Purpose of the Study:
- To investigate the impact of deciduous and permanent dentine extracts on osteoclast activity.
- To determine if dentine composition influences the molecular mechanisms of root resorption.
Main Methods:
- Osteoclasts were generated from mouse bone marrow cells.
- Osteoclasts were incubated with hydrochloric acid (HCl) extracts from bovine deciduous or permanent dentine.
- Osteoclast activity markers including TRAP activity, resorption pit area, and mRNA levels of key genes (TRAP, v-ATPase, calcitonin receptor, cathepsin K, MMP-9) were analyzed.
Main Results:
- Both deciduous and permanent dentine extracts appeared to suppress osteoclast activity, evidenced by reduced TRAP activity and resorption areas.
- mRNA levels of osteoclast marker genes were also suppressed by the dentine extracts.
- While not statistically significant, permanent dentine extracts showed a trend towards greater suppression of osteoclast resorption compared to deciduous dentine extracts.
Conclusions:
- Both deciduous and permanent dentine contain factors that can inhibit osteoclast activity.
- The study suggests a potential, albeit not fully elucidated, difference in the inhibitory effects of permanent versus deciduous dentine on osteoclast-mediated resorption.
Related Concept Videos
01:31Osteoclasts in Bone Remodeling
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
01:18The Bone Matrix
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in acid or...
01:40Bone Remodeling
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
01:23Role of Matrix Metalloproteases in Degradation of ECM
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
A...
01:17Hormones and Bone Tissue
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
01:29Bone Disorders
Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...