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Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
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Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Related Experiment Video

Updated: Jun 24, 2026

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Chromatin dynamics is correlated with replication timing.

Artem Pliss1, Kishore Malyavantham, Sambit Bhattacharya

  • 1Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, NY 14260, USA.

Chromosoma
|March 20, 2009
PubMed
Summary

Chromatin dynamics, the movement of DNA structures (chromatin domains), are highest during early cell division (S-phase). This suggests active gene transcription is linked to dynamic chromatin organization.

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14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Discrete chromatin domains (ChrD) are fundamental units regulating DNA organization and replication.
  • Understanding chromatin dynamics is crucial for comprehending gene regulation and cellular processes.

Purpose of the Study:

  • To measure the translational motion of chromatin domains in living cells using a bio-computational approach.
  • To investigate the relationship between chromatin dynamics, replication timing, and transcriptional activity.

Main Methods:

  • Utilized a bio-computational method to simultaneously track the movement of large populations of chromatin domains (ChrD) in live cell nuclei.
  • Assessed the impact of transcription inhibitors (alpha-amanitin and actinomycin D) on chromatin dynamics.
  • Analyzed the colocalization of ChrD with transcription sites and complementary DNA (cDNA).

Main Results:

  • Chromatin domains replicating in early S-phase exhibited significantly higher movement and configurational changes compared to those replicating later.
  • Chromatin dynamics were unaffected by transcription inhibition with alpha-amanitin.
  • Actinomycin D treatment substantially reduced chromatin dynamics.
  • A correlation was observed between higher chromatin dynamics and chromatin poised for active transcription, particularly in early S-phase.

Conclusions:

  • Chromatin dynamics are closely linked to replication timing, with early S-phase domains being more dynamic.
  • The findings suggest a correlation between chromatin dynamics and the potential for active gene transcription.
  • Chromatin organization and dynamics play a significant role in regulating gene expression during the cell cycle.