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Published on: June 9, 2021
Rapid screening for anthocyanins and anthocyanin dimers in crude grape extracts by high performance liquid
Sandra Pati1, Maria Teresa Liberatore, Giuseppe Gambacorta
1Department of Food Science, University of Foggia-Via Napoli 25, Foggia, Italy. s.pati@unifg.it
Abstract:
A rapid and efficient method using high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry (HPLC-DAD-MS/MS) for fast screening large numbers of anthocyanins and anthocyanin dimers in different grape skin extracts, without further sample clean-up procedures, was developed. A good separation of most detected anthocyanins was achieved in a run time of 15 min. Identification of anthocyanin pigments required a combination of several information: UV-vis spectra, MS and MS/MS spectra, and elution pattern. Many compounds have been here detected for the first time and their structures tentatively elucidated.
