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Published on: July 15, 2011
DNA typing using AmpFlSTR Y-filer for very long-term stored specimens
Kazuhiko Tsukada1, Maiko Shimizu, Yoshinobu Kurasawa
1Department of Forensic Medicine, Criminal Investigation Laboratory, Nagano Pref. Police H.Q., 3916 Nishijo, Matsushiro-Machi, Nagano-shi, Nagano 381-1232, Japan. tuk-lab@mx1.avis.ne.jp
Abstract:
Aware that long-term storage bloodstain generally reduces numbers of detectable loci, we performed DNA typing with bloodstains stored at room temperature for 22-30years, then extracted DNA from the bloodstains using the AmpFlSTR Y-filer PCR Amplification Kit. We performed electrophoresis with an ABI 310 Genetic Analyzer and determined alleles using GeneMapper ID v3.2 software. Our results suggest that Y-filer finds certain loci more difficult to anneal than others.

