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Related Concept Videos

Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...

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Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
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Assessing tumor angiogenesis in histological samples.

Stephen B Fox1

  • 1Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|March 24, 2009
PubMed
Summary

Tumor neovascularization involves complex vessel formation processes. Histological assessment of tumor vascularity, while mainly for research, shows potential for clinical applications with proper methods.

Area of Science:

  • Oncology
  • Vascular Biology

Background:

  • Tumor neovascularization is crucial for tumor growth, involving angiogenesis, vasculogenesis, and other processes.
  • Quantifying tumor vasculature is essential for understanding tumor biology and progression.

Purpose of the Study:

  • To review the methods for assessing tumor vascularity.
  • To highlight the molecular pathways and reagents used to study tumor neovascularization.
  • To discuss the potential clinical applications of histological assessment of tumor vascularity.

Main Methods:

  • Immunohistochemical staining to identify microvessels.
  • Chalkley counting and image analysis systems for objective quantification.
  • Utilizing reagents targeting angiogenic factors, cell adhesion molecules, proteases, and vessel activation markers (e.g., CD105).

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Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells
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Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Published on: November 23, 2014

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Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Published on: November 23, 2014

Main Results:

  • Various methods exist for quantifying tumor vasculature, with image analysis offering objectivity.
  • Numerous molecular pathways and reagents are available for studying tumor neovascularization.
  • Distinguishing between quiescent and active vessels, and identifying hypoxia-related proteins, are also possible.

Conclusions:

  • Histological assessment of tumor vascularity is a valuable research tool.
  • Standardized methodology and trained observers could enable clinical applications of tumor vascularity assessment.