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On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
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Multiplex polymerase chain reaction (PCR) and real-time multiplex PCR for the simultaneous detection of plant viruses
V Pallas1, J Sanchez-Navarro, A Varga
1IBMCP, Universidad Politecnica de Valencia-Consejo Superior de Investigaciones Cientificas, CPI, Valencia, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|March 24, 2009
Abstract:
Multiplex Polymerase Chain Reaction (PCR) can be used for the simultaneous detection of plant viruses. Multiple primer pairs or polyvalent primer pairs can be used to detect and identify several viruses in a single PCR.
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02:57Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
01:32PCR - Polymerase Chain Reaction
Overview