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On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
Published on: February 23, 2018
Direct detection of plant viruses in potato tubers using real-time PCR
Neil Boonham1, Lynn Laurenson, Rebecca Weekes
1Central Science Laboratories, Sand Hutton, York, UK.
Methods in Molecular Biology (Clifton, N.J.)
|March 24, 2009
Abstract:
Virus indexing of seed potatoes can be carried out by growing eye plugs to produce small plants and then testing them by ELISA, but this method is time consuming. Direct testing of the eye plugs by ELISA is not reliable, and so a method has been developed for the routine testing of seed potatoes for virus by PCR.
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