Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA repair drives cisplatin-induced neuronal death.

Cell·2026
Same author

Uridine cytidine kinases govern molnupiravir bioactivation and anti-SARS-CoV-2 activity.

PLoS pathogens·2026
Same author

In-situ ion irradiation investigations on MBE grown Sb thin films on sapphire.

Scientific reports·2026
Same author

The modifiers that cause changes in gene essentiality.

Cell systems·2026
Same author

Mislabeled and Misunderstood: Large Mammal Distribution Underscores Ecological Significance of Agro-Pastoral "Wastelands" in India's Deccan Peninsula.

Ecology and evolution·2026
Same author

Targeting IMPDH to inhibit SAMHD1 in <i>KMT2A</i>-rearranged leukaemia.

Cell cycle (Georgetown, Tex.)·2025

Related Experiment Video

Updated: Jun 24, 2026

Microarray Analysis for Saccharomyces cerevisiae
13:17

Microarray Analysis for Saccharomyces cerevisiae

Published on: April 7, 2011

Total RNA isolation from recalcitrant yeast cells.

M Amin-ul Mannan1, Sushma Sharma, K Ganesan

  • 1Institute of Microbial Technology (Council of Scientific and Industrial Research), Sector 39-A, Chandigarh 160036, India.

Analytical Biochemistry
|March 24, 2009
PubMed
Summary

A new, easy method isolates high-quality RNA from difficult yeast cells, ideal for processing many samples quickly for applications like quantitative real-time polymerase chain reaction.

More Related Videos

DNAzyme-dependent Analysis of rRNA 2&#8217;-O-Methylation
09:12

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Related Experiment Videos

Last Updated: Jun 24, 2026

Microarray Analysis for Saccharomyces cerevisiae
13:17

Microarray Analysis for Saccharomyces cerevisiae

Published on: April 7, 2011

DNAzyme-dependent Analysis of rRNA 2&#8217;-O-Methylation
09:12

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Conventional RNA isolation methods are ineffective for stationary-phase yeast and fermentation broths.
  • Existing specialized methods for recalcitrant yeast are often complex and not suitable for high-throughput sample processing.

Purpose of the Study:

  • To develop a facile and efficient RNA isolation method for recalcitrant yeast cells.
  • To provide a high-throughput compatible protocol for obtaining quality RNA from yeast.

Main Methods:

  • A novel RNA isolation procedure performed entirely within microcentrifuge tubes.
  • The method is designed for rapid processing of multiple yeast samples.

Main Results:

  • Consistent isolation of high-quality and high-quantity RNA from challenging yeast sources.
  • The isolated RNA is suitable for downstream molecular analyses, including quantitative real-time polymerase chain reaction (qRT-PCR).
  • The method demonstrates broad applicability across different yeast species, including Saccharomyces cerevisiae.

Conclusions:

  • The developed method offers a significant improvement for RNA isolation from recalcitrant yeast.
  • Its efficiency and suitability for high-throughput applications make it valuable for yeast research and biotechnology.
  • The protocol's versatility across species suggests widespread utility in various yeast-based studies.