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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Nitric oxide inhibitory principles from Derris trifoliata stems.
Supinya Tewtrakul1, Sarot Cheenpracha, Chatchanok Karalai
1Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. supinyat@yahoo.com
Summary
Derris trifoliata stems yield potent nitric oxide (NO) inhibitors, with 12a-hydroxyrotenone showing exceptional activity. Certain rotenoids also exhibit significant antioxidant properties, with no observed cytotoxicity at effective concentrations.
Area of Science:
- Natural Product Chemistry
- Pharmacology
- Medicinal Chemistry
Background:
- Derris trifoliata is a plant source of rotenoids.
- Nitric oxide (NO) plays a role in various physiological and pathological processes.
- Antioxidant activity is crucial for combating oxidative stress.
Purpose of the Study:
- To isolate and characterize rotenoids from Derris trifoliata stems.
- To evaluate the nitric oxide inhibitory activity of isolated rotenoids.
- To assess the DPPH radical scavenging activity of these compounds.
Main Methods:
- Extraction of plant material using hexane and dichloromethane.
- Isolation and purification of nine rotenoids.
- In vitro testing of NO inhibitory activity using RAW264.7 cells.
- Evaluation of DPPH radical scavenging activity.
- Cytotoxicity assessment.
Main Results:
- 12a-hydroxyrotenone (7) demonstrated potent NO inhibition (IC50=0.002 microM).
- Deguelin (1) and 12a-hydroxyelliptone (9) also showed significant NO inhibitory activity.
- 6a,12a-dehydrodeguelin (5) exhibited the highest DPPH scavenging activity (IC50=7.4 microM).
- No cytotoxicity was observed for the tested compounds at their effective concentrations.
Conclusions:
- The isolated rotenoids from Derris trifoliata stems are responsible for the observed NO inhibitory and antioxidant effects.
- Structural modifications like hydroxylation and prenylation significantly influence NO inhibitory activity.
- The presence of a double bond at C6a-C12a enhances DPPH scavenging activity.
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