Related Experiment Video
Updated: Jun 24, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Published on: July 9, 2021
Reducing the NMR sample volume using a single organic liquid: increased sensitivity for mass-limited samples with
1Institute for Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, ON, Canada K1A 0R6. Harold.Jarrell@nrc-cnrc.gc.ca
Abstract:
A simple inexpensive protocol for confining an aqueous sample to the active region of a standard NMR probe is examined for high-resolution NMR. The aqueous sample is sandwiched between an inert perfluorinated organic liquid that has been exploited in the design of micro-coil NMR probes. The procedure is demonstrated with 3mm NMR tubes at ambient and elevated temperatures but should be equally applicable to smaller diameter tubes. It is shown that confinement has minimal effects on line shape and provides at least a two fold increase in sensitivity over a conventional sample, for the same mass of solute.
Related Concept Videos
Chemical Shift: Internal References and Solvent Effects
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
NMR Spectrometers: Resolution and Error Correction
¹H NMR Signal Integration: Overview
Double Resonance Techniques: Overview
Spin decoupling is usually achieved by...
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
¹H NMR of Labile Protons: Deuterium (²H) Substitution

