Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Redesign of energetically frustrated regions rescues function in defective T4 clamp loaders.

bioRxiv : the preprint server for biology·2026
Same author

The scientific legacy of Martin Karplus from the perspective of his collaborators.

Biophysical journal·2026
Same author

Altered residence time as a cause of drug resistance.

Current opinion in structural biology·2026
Same author

BTK autoinhibition analyzed by high-throughput swaps of SH2 domains.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Autophosphorylation of oncoprotein TEL-ABL in myeloid and lymphoid cells confers resistance to the allosteric ABL inhibitor asciminib.

Science signaling·2025
Same author

Resistance to Allosteric Inhibitors.

Journal of molecular biology·2025
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

A MAPK scaffold lends a helping hand.

Markus A Seeliger1, John Kuriyan

  • 1Department of Molecular and Cell Biology, University of California, Howard Hughes Medical Institute, Berkeley, Berkeley, CA 94720, USA.

Cell
|March 24, 2009
PubMed
Summary
This summary is machine-generated.

Scaffold proteins like Ste5 are not passive. In budding yeast, Ste5 actively allosterically activates the Fus3 kinase, enabling signal transmission in this crucial mitogen-activated protein kinase pathway.

More Related Videos

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
10:43

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

Microfluidic Synthesis of Microgel Building Blocks for Microporous Annealed Particle Scaffold
09:34

Microfluidic Synthesis of Microgel Building Blocks for Microporous Annealed Particle Scaffold

Published on: June 16, 2022

Related Experiment Videos

Last Updated: Jun 24, 2026

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
10:43

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

Microfluidic Synthesis of Microgel Building Blocks for Microporous Annealed Particle Scaffold
09:34

Microfluidic Synthesis of Microgel Building Blocks for Microporous Annealed Particle Scaffold

Published on: June 16, 2022

Area of Science:

  • Cellular signaling pathways
  • Molecular biology
  • Yeast genetics

Background:

  • Scaffold proteins traditionally viewed as passive organizers of signaling cascades.
  • Mitogen-activated protein kinase (MAPK) pathways are critical for cellular communication.
  • The role of scaffold proteins in regulating kinase activity remains an area of investigation.

Purpose of the Study:

  • To investigate the functional role of the scaffold protein Ste5 in budding yeast.
  • To determine if Ste5 possesses active regulatory functions beyond simple scaffolding.
  • To elucidate the mechanism by which Ste5 influences the activity of the Fus3 kinase.

Main Methods:

  • Genetic analysis in Saccharomyces cerevisiae (budding yeast).
  • Biochemical assays to assess kinase activity and substrate recognition.
  • Studies focusing on the interaction between Ste5 and Fus3.

Main Results:

  • Demonstrated that the scaffold protein Ste5 is an allosteric activator of the Fus3 kinase.
  • Showed that Ste5 binding renders Fus3 competent as a kinase substrate.
  • Provided evidence against the passive tethering model for Ste5 function.

Conclusions:

  • Scaffold proteins can play active, regulatory roles in signaling pathways.
  • Ste5 actively modulates Fus3 kinase activity, facilitating signal transmission.
  • This finding redefines the functional paradigm of scaffold proteins in cellular signaling.