Related Experiment Video
Updated: Jun 24, 2026
10:46
Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
Simple technique for graft insertion in Descemet-stripping (automated) endothelial keratoplasty using a 30-gauge
Chandra Balachandran1, Lisanne Ham, Rénuka S Birbal
1Netherlands Institute for Innovative Ocular Surgery, Rotterdam, The Netherlands.
Journal of Cataract and Refractive Surgery
|March 24, 2009
Abstract:
We describe a needle insertion technique for graft insertion in Descemet-stripping (automated) endothelial keratoplasty (DSEK/DSAEK). A folded donor posterior corneal disk is inserted through a 5.0 mm scleral tunnel incision over a plastic glide using a 30-gauge needle. The technique enables safe and easy graft insertion without vertical or horizontal compression of the donor tissue, causing minimal trauma to the donor endothelium and/or host structures.
