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Compact Quantum Dots for Single-molecule Imaging
Published on: October 9, 2012
Synthesis and characterization of double-layer quantum-dots-tagged microspheres
Xinghua Pan1, Maolin Lu, Daocheng Wu
1Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
IEEE Transactions on Nanobioscience
|March 24, 2009
Summary
Synthesized quantum-dots-tagged poly (styrene-acrylamide-acrylic acid) microspheres (QDsAAMs) feature active azidocarbonyl groups for protein immobilization. These QDsAAMs offer sensitive fluorescence for potential use in diagnostics.
Area of Science:
- Materials Science
- Bioconjugation Chemistry
- Nanotechnology
Background:
- Development of novel fluorescent probes is crucial for sensitive biomolecule detection.
- Surface modification of microspheres enables targeted functionalization for various applications.
- Azidocarbonyl chemistry offers efficient and mild bioconjugation strategies.
Purpose of the Study:
- To synthesize and characterize quantum-dots-tagged poly (styrene-acrylamide-acrylic acid) microspheres (QDsAAMs).
- To introduce azidocarbonyl groups onto QDsAAMs for protein immobilization.
- To evaluate the potential of functionalized QDsAAMs as fluorescent probes for diagnostics.
Main Methods:
- Synthesis of QDsAAMs via polymerization and tagging with quantum dots.
- Surface modification using hydrazinolysis followed by azido reaction to introduce azidocarbonyl groups.
- Characterization using microscopy, spectroscopy, and size analysis.
- Bovine serum albumin (BSA) immobilization and fluorescence quantification.
Main Results:
- QDsAAMs exhibited a regular double-layer spherical structure with an average diameter of 11.2 microm.
- High fluorescence intensity (lambda(ex)/lambda(em) = 250 nm/370 nm) with linearity over a concentration range of 3.0 x10(-3) to 90.0 x10(-3) g.L(-1).
- Stable fluorescence characteristics were observed under varying pH and ionic strength.
- Successful covalent immobilization of BSA onto azidocarbonyl QDsAAMs at a rate of 40 x10(-3) g/g, with preserved fluorescence linearity.
Conclusions:
- Functional azidocarbonyl QDsAAMs possess sensitive fluorescence and active azidocarbonyl groups.
- These microspheres are suitable for quantitative detection and protein immobilization.
- The developed QDsAAMs show promise as fluorescent probes for early and rapid clinical diagnostics.
