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Updated: Jun 24, 2026

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In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
Published on: August 6, 2025
Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system
1School of Biotechnology and Biomolecular Sciences, University of New South Wales, Randwick, NSW 2052, Australia. b.ferrari@unsw.edu.au
Applied and Environmental Microbiology
|March 24, 2009
Abstract:
Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

