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Related Concept Videos

Colloidal precipitates01:09

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jun 24, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Surface-adsorbed silver half-shells as a platform for surface-enhanced immunoassays; optimization through

Tetsuji Yamaguchi1, Takatoshi Kaya, Misao Aoyama

  • 1Fujirebio Inc., 51 Komiya, Hachioji, Tokyo, Japan.

The Analyst
|March 24, 2009
PubMed
Summary

Silver half-shells on latex spheres boost surface-enhanced fluorescence. Optimizing sphere diameter, silver thickness, and density significantly increases fluorescence signals for potential immunoassay applications.

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Area of Science:

  • Nanotechnology
  • Surface chemistry
  • Optical physics

Background:

  • Surface-enhanced fluorescence (SEF) is crucial for sensitive bioassays.
  • Silver nanostructures are effective platforms for SEF.
  • Optimizing nanostructure geometry and density is key to maximizing SEF.

Purpose of the Study:

  • To investigate silver half-shells on latex spheres for SEF.
  • To optimize physical parameters for enhanced fluorescence.
  • To assess the potential for immunoassay applications.

Main Methods:

  • Fabrication of silver-coated latex spheres with controlled parameters.
  • Systematic variation of latex sphere diameter, silver deposition thickness, and adsorption density.
  • Measurement of fluorescence enhancement using surface-adsorbed silver half-shells.

Main Results:

  • Maximum fluorescence enhancement of 25.5 achieved with 56 nm spheres and 10 nm silver.
  • Optimal sparse adsorption density (12.5 per µm²) yielded higher signals than dense coverage (105.9 per µm²).
  • Further threefold increase in enhancement observed after optimizing adsorption density.

Conclusions:

  • Surface-adsorbed silver half-shells are effective for SEF.
  • Optimized sphere size, silver thickness, and adsorption density significantly enhance fluorescence.
  • The developed platform shows promise for sensitive immunoassay development.