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Cell Lines

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

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Updated: Jun 24, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

Automation of cell line development.

Kristina Lindgren¹, Andréa Salmén, Mats Lundgren

¹BioProcess R&D, AstraZeneca (now Recipharm Biologics AB), Gärtunavägen 10, 152 57, Södertälje, Sweden.

|March 24, 2009

Summary

Automated cell line development for biopharmaceuticals increases throughput and reduces costs. This robotic system produces high-quality cell lines comparable to manual methods, enhancing production capacity.

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Area of Science:

Biopharmaceutical Manufacturing
Cell Line Development
Process Automation

Background:

Biopharmaceutical production relies on high-producing cell lines, a process traditionally limited by throughput and cost.
Automation offers a solution to increase efficiency and reduce development timelines.

Purpose of the Study:

To evaluate an automated platform for developing high-producing cell lines for monoclonal antibody production.
To assess the quality and efficiency of automated cell line development compared to manual methods.

Main Methods:

Utilized the Cello robotic system for screening and expansion of Chinese hamster ovary (CHO) cell cultures.
Employed the glutamine synthetase expression system for monoclonal antibody production.
Investigated the impact of methionine sulphoximine (MSX) concentrations on colony selection.

Main Results:

Automated platform yielded cell lines of comparable quality to traditional methods in terms of colony detection and IgG titer distribution.
Best expressing cell lines achieved IgG titers of 4.7 and 5.0 g/L in fed-batch bioreactor evaluations.
Higher MSX concentration (75 µM) reduced low-producing transfectants while maintaining high-producing cell lines, increasing parallel project capacity.

Conclusions:

The automated platform significantly increases cell line development project capacity (up to threefold) without increasing manual workload.
Automation ensures cell line quality and enhances overall biopharmaceutical production efficiency.
Optimizing selection agent concentration in automated systems can further improve capacity and efficiency.