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Updated: Jun 24, 2026

Volatile Sex Pheromone Extraction and Chemoattraction Assay in Caenorhabditis elegans
Published on: August 9, 2024
Chemotactic responses of male Caenorhabditis elegans
1Schools of Biology and Physics, Georgia Institute ofTechnology, Atlanta, GA 30332, USA.
Abstract:
Cultures of C. elegans containing a high proportion of males were subjected to chemotactic tests by using the method of countercurrent separation. The responses of males and hermaphrodites were determined. Both types of worms preferred Na over 1/2 Ca(2), Cl over NO; they were attracted to NaCl, OH, cyclic AMP, pyridine, CO, in borate buffer (pH 8.8); and avoided CO in phosphate buffer (pH 6.0), D-tryptophan, and acid. It was thus concluded that male C. elegans have the same chemotactic responses that hermaphrodites of this species are known to have.

