Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Condensins02:15

Condensins

Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to: Assessing SMC Complex Function in Replication Fork Progression with DNA Fiber Assays.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

High-Throughput Proteomics Sample Preparation Using a 96-Channel Pipettor and Magnetic Pin Device.

Journal of proteome research·2026
Same author

Author Correction: Mcm10 associates with the loaded DNA helicase at replication origins and defines a novel step in its activation.

The EMBO journal·2025
Same author

Eukaryotic Replisome Components Cooperate to Process Histones During Chromosome Replication.

Cell reports·2025
Same author

Visualizing Binding of Single SMC Complexes to DNA Substrates Using Combinational Optical Tweezers and Fluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Assessing SMC Complex Function in Replication Fork Progression with DNA Fiber Assays.

Methods in molecular biology (Clifton, N.J.)·2025

Related Experiment Video

Updated: Jun 24, 2026

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples
05:45

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples

Published on: May 3, 2024

The unnamed complex: what do we know about Smc5-Smc6?

Giacomo De Piccoli1, Jordi Torres-Rosell, Luis Aragón

  • 1Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College, Du Cane Road, London W12 0NN, UK.

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
|March 25, 2009
PubMed
Summary

The Smc5-Smc6 complex, involved in DNA repair and chromosome stability, is crucial for sister chromatid recombination. Its functions, including SUMOylation, are key to maintaining genomic integrity.

More Related Videos

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Related Experiment Videos

Last Updated: Jun 24, 2026

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples
05:45

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples

Published on: May 3, 2024

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Structural Maintenance of Chromosome (SMC) proteins form core complexes: cohesin, condensin, and Smc5-Smc6.
  • Cohesin and condensin roles in sister chromatid cohesion and chromosome condensation are established.
  • The cellular function of the Smc5-Smc6 complex remains largely uncharacterized.

Purpose of the Study:

  • To review the current understanding of the Smc5-Smc6 protein complex.
  • To elucidate the cellular functions and significance of Smc5-Smc6 in DNA repair and chromosome stability.

Main Methods:

  • Literature review of studies on Smc5-Smc6 complex.
  • Analysis of Smc5-Smc6 involvement in DNA repair pathways.
  • Examination of Smc5-Smc6 mutant phenotypes and associated activities.

Main Results:

  • Smc5-Smc6 is implicated in various DNA repair pathways.
  • The complex exhibits DNA helicase and endonuclease activities.
  • Smc5-Smc6 is essential for sister chromatid recombination; mutants accumulate recombination intermediates.
  • The complex possesses SUMO-ligase and potential ubiquitin-ligase activities.

Conclusions:

  • Despite its unclear name, Smc5-Smc6 is vital for chromosome stability.
  • The complex likely plays a significant role in maintaining genomic integrity through DNA repair and recombination regulation.