Related Experiment Video
Updated: Jun 24, 2026

Surgical Injury to the Mouse Pancreas through Ligation of the Pancreatic Duct as a Model for Endocrine and Exocrine Reprogramming and Proliferation
Published on: August 7, 2015
Increased ribosomal biogenesis induces pancreatic beta cell failure in mice model of type 2 diabetes
Shun-ichiro Asahara1, Tomokazu Matsuda, Yoshiaki Kido
1Department of Internal Medicine, Division of Diabetes, Metabolism, and Endocrinology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Aim:
To study the changes in gene expression by pancreatic beta cells under insulin resistance conditions.
Method:
An exhaustive gene expression analysis was performed, using isolated pancreatic islets of obese diabetic model Lepr(-/-) mice. Overexpression of cyclin D2 was induced in cells from the pancreatic beta cell line, namely, INS-1.
Results:
Through a gene expression analysis using islets isolated from db/db mice, we found a significant increase in the expression of ribosome-related molecules. In addition, increased expression of cyclin D2 was found at certain protein levels. As INS-1 cells were induced to overexpress cyclin D2, we found an increase in the expression of ribosome-related molecules. Concurrently, an increase in the expression of endoplasmic reticulum stress (ER stress)-related molecules was also found.
Conclusion:
In cases of pancreatic beta cell hyperplasia associated with insulin resistance, ribosomal biogenesis is increased, and ER stress is induced.
Related Concept Videos
Type II Diabetes II: Pathophysiology
Type I Diabetes II: Pathophysiology
Insulin: Biosynthesis, Chemistry, and Preparation
Damage or functional impairment of β-cells inhibits insulin production, leading to diabetes. Diabetes treatment primarily uses...
Type II Diabetes I: Introduction
Insulin Secretory Vesicles
Chronic Pancreatitis II: Pathophysiology
