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PCR-mediated chemical mutagenesis of cloned duplex DNAs
J J Diaz1, D D Rhoads, D J Roufa
1Division of Biology, Kansas State University, Manhattan 66506.
Biotechniques
|August 1, 1991
Abstract:
We describe an efficient, PCR-mediated protocol for random chemical mutagenesis of cloned duplex DNAs. The method involves a single molecular cloning step and is compatible with a wide variety of recombinant DNA vectors. To illustrate the procedure, we report the nitrous acid mutagenesis of a human ribosomal protein S14 cDNA fragment.