Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Cytosolic Zinc Finger Domain Structure of the CCHFV Glycoprotein n Is Maintained in Its Membrane-Bound Form.

Journal of the American Chemical Society·2026
Same author

An engineered closed-shell, two-component, 480-subunit nucleocapsid.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

NMR Structural Characterization of SARS-CoV-2 ORF6 Reveals an N-Terminal Membrane Anchor.

Journal of the American Chemical Society·2025
Same author

Analysis of the structure and interactions of the SARS-CoV-2 ORF7b accessory protein.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Hepatitis Delta Antigen Retains the Assembly Domain as the Only Rigid Entity.

Journal of the American Chemical Society·2024
Same author

A structural rationale for reversible vs irreversible amyloid fibril formation from a single protein.

Nature communications·2024
Same journal

Anharmonic phonons via quantum thermal bath simulations.

The Journal of chemical physics·2026
Same journal

Quantum simulation of alignment dependent differential cross sections in co-propagating molecular beams at cold collision energies.

The Journal of chemical physics·2026
Same journal

Non-additive ion effects on the coil-globule equilibrium of a generic polymer in aqueous salt solutions.

The Journal of chemical physics·2026
Same journal

Insights into the unexpected small reduction of the temperature of maximum density of water by lithium chloride addition.

The Journal of chemical physics·2026
Same journal

Optical frequency comb double-resonance spectroscopy of the 9030-9175 cm-1 states of ethylene.

The Journal of chemical physics·2026
Same journal

Time reversal breaking of colloidal particles in cells.

The Journal of chemical physics·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Understanding two-pulse phase-modulated decoupling in solid-state NMR.

Ingo Scholz1, Paul Hodgkinson, Beat H Meier

  • 1Physical Chemistry, ETH Zurich, 8093 Zurich, Switzerland.

The Journal of Chemical Physics
|March 26, 2009
PubMed
Summary
This summary is machine-generated.

This study presents a theoretical description of two-pulse phase-modulated (TPPM) decoupling in magic-angle spinning NMR. The advanced Floquet approach reveals that TPPM decoupling can significantly reduce linewidths in solid-state NMR spectroscopy.

More Related Videos

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Related Experiment Videos

Last Updated: Jun 24, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Solid-State NMR
  • Quantum Information Science

Background:

  • Magic-angle spinning (MAS) NMR is crucial for high-resolution solid-state structural analysis.
  • Decoupling techniques are essential for suppressing unwanted spin-spin interactions and narrowing spectral lines.
  • Existing theoretical frameworks for decoupling sequences have limitations in describing complex parameter spaces.

Purpose of the Study:

  • To provide a comprehensive theoretical description of the two-pulse phase-modulated (TPPM) decoupling sequence in MAS NMR.
  • To develop analytical expressions for residual coupling and spin-diffusion terms.
  • To characterize resonance conditions affecting linewidths and advance understanding of decoupling mechanisms.

Main Methods:

  • Utilizing a triple-mode Floquet approach for theoretical analysis.
  • Formulating the description in the radio-frequency interaction-frame representation.
  • Validating the approach across the full range of TPPM parameters.

Main Results:

  • Derived analytical expressions for heteronuclear residual coupling and homonuclear spin-diffusion terms.
  • Identified and characterized resonance conditions influencing residual linewidths, including novel ones.
  • Demonstrated that a combined Floquet description of residual couplings and resonance conditions is necessary for accurate decoupling behavior prediction.
  • Achieved a theoretical (13)C linewidth of approximately 12 Hz for TPPM decoupling in organic solids or proteins.

Conclusions:

  • The triple-mode Floquet approach provides a robust theoretical framework for TPPM decoupling in MAS NMR.
  • TPPM decoupling significantly contributes to linewidth reduction, approaching experimental limits.
  • Decoupling techniques remain a critical factor influencing achievable linewidths in solid-state NMR, highlighting areas for further optimization.