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Updated: Jun 24, 2026

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
Published on: June 26, 2019
High-throughput verification of transcriptional starting sites by Deep-RACE
Signe Olivarius1, Charles Plessy, Piero Carninci
1Functional Genomics Technology Team, Omics Science Center, RIKEN Yokohama Institute, Kanagawa, Japan.
Abstract:
We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep-rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.
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