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Updated: Jun 24, 2026

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
Published on: April 24, 2020
Preparation of plasma samples for chromatographic analyses using functionalized ferromagnetic micro-particles
Michael Vogeser1, Albert Geiger, Rupert Herrmann
1Institute of Clinical Chemistry, Hospital of the University of Munich, Marchioninistr. 15, D-81377 Munich, Germany. Michael.Vogeser@med.uni-muenchen.de
Objectives:
To study if functionalized ferromagnetic micro-particles used for extraction procedures prior to chromatographic analyses can be handled within an HPLC system.
Method:
We used C18-functionalized ferromagnetic micro-particles, manipulated within a high-performance liquid chromatography system for extraction of plasma samples, with a novel extractor device enabling reversible immobilization of the particles within HPLC tubing. A pilot analytical system for the quantification of itraconazole in plasma--as a representative small molecule analyte--by ultraviolet detection was configured and tested.
Results:
The system allowed linear and reproducible quantification of the representative target analyte.
Conclusions:
Sample preparation of biological samples can be achieved by handling of functionalized ferromagnetic micro-particles which are handled within a high pressure liquid chromatography configuration; this offers interesting perspectives for the development of automated sample preparation devices.
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